Assuntos
Humanos , Células Epiteliais , Cílios , Células Cultivadas , Metronidazol/farmacocinética , PneumopatiasAssuntos
Edema/diagnóstico , Pólipos Nasais/diagnóstico , Doenças Nasais/diagnóstico , Rinoscleroma/diagnóstico , Anosmia/diagnóstico , Anosmia/patologia , Edema/patologia , Edema/terapia , Dor Facial/diagnóstico , Dor Facial/patologia , Feminino , Febre/diagnóstico , Febre/patologia , Cefaleia/diagnóstico , Cefaleia/patologia , Humanos , Pessoa de Meia-Idade , Mucosa Nasal/patologia , Pólipos Nasais/patologia , Pólipos Nasais/terapia , Doenças Nasais/patologia , Doenças Nasais/terapia , Rinoscleroma/patologia , Rinoscleroma/terapiaAssuntos
Amebíase/diagnóstico , Amebíase/patologia , Urina/citologia , Adulto , Humanos , Masculino , Adulto JovemRESUMO
PURPOSE: The aim of this study was to investigate protozoan cysts and oocyts in the faecal pellets of the German cockroach (Blattella germanica), with emphasis on the prevalence of Lophomonas blattarum. METHODS: Thirty adult Blattella germanica (12 males; 18 females) were trapped near Madrid, Spain. Expelled faecal pellets were collected in the laboratory over 5 days in two plastic containers. Protozoan cysts from one container were weighted and those in the other used for morphologically identification. RESULTS: Protozoan cysts/oocysts per gram of faecal pellet were: Nyctotherus sp. (0.0019/g), Entamoeba (0.0007/g), Balantidium coli (0.0001/g), Lophomonas blattarum (0.00038/g). Observation of 189 protozoan cysts/oocysts indicated the following prevalence: Nyctotherus sp. 65 (34.4%); Gregarina spp. 34 (18%); Entamoeba sp. 24 (12.7%); Cryptosporidium sp. 17 (9%); Coccidia 16 (8.4%); Lophomonas blattarum 13 (6.8%); Balantidium coli 4 (2.1%); and unclassified 16 (8.4%). CONCLUSION: Lophomonas blattarum has previously been demonstrated in the gut of cockroaches, but this is the first assessment of the prevalence in Blattella germanica in faecal pellets. The presence of protozoa in faecal pellets provides some evidence for one step in a hypothesised route of respiratory infection whereby protozoa from household insects enter the respiratory tract.
Assuntos
Blattellidae , Baratas , Criptosporidiose , Cryptosporidium , Cistos , Parabasalídeos , Adulto , Animais , Feminino , Humanos , MasculinoRESUMO
Human rhinovirus infection has been identified as the commonest cause of common cold. We were therefore interested in cytological assessment of nasal cells from patients with human rhinovirus infection. Cytological examination was undertaken of nasal smears from a group of 7 patients, 4 females and 3 males; aged between 27 and 14 years-old (average = 17.5), who had longstanding perennial rhinitis (in all cases 7 years or more). They presented at our research unit showing clinical symptoms of a common cold, including sore throat, nasal congestion, sneezing, and watery rhinorrhea. Serology and DNA real-time PCR demonstrated human rhinoviruses type A (5 cases) and C (two cases) as the cause of the infection. Nasopharyngeal swabs were taken, fixed with 96% ethanol and stained using the Papanicolaou method and Giemsa stain. Under light microscopy the Pap smears showed the presence of numerous detached ciliated nasal epithelial cells, with approximately one third of the cells showed destruction and irregular attachment of cilia, microvacuolated cytoplasm, and large red round intranuclear bodies, similar to inclusions, surrounded by clear halos, where the nuclear borders were visible. Ultrastructural examination of the cells indicated disappearance of cilia, or shortened, fragmented and disordered cilia. Large intranuclear bodies appeared as a compact mass similar, similar to the condensed chromatin, and separated from the nuclear membrane by a clear space (Figure 1B). No viral particles were observed in the cytoplasm or nucleus. Our results confirm that human rhinovirus can alter the morphology of the ciliated nasal epithelial cells, principally causing alterations to the cilia and provoking nuclear changes.
